===== LB (Luria Broth) 500mL =====
|Trypton|5.0 g|
|Yeast Extract|2.5 g|
|NaCl|5.0 g|


===== LB Plate (solid) 300mL =====
|Trypton|3.0 g|
|Yeast Extract|1.5 g|
|NaCl|3.0 g|
|Agar|4.5 g|


Appropriate amount of antibiotics: 100 µg/mL Amp,  50 µg/mL Kan

===== M9 (15N, 1 L) =====
[[https://wasatch.biochem.utah.edu/nmr/M9media.pdf|ref1]] [[http://www.thelabrat.com/protocols/m9minimal.shtml|ref2]]
|Na<sub>2</sub>HPO<sub>4</sub>|12.8 g|
|KH2PO4|3.0g|
|NaCl|0.5g|
|15N-NH4Cl|1g|
|  Add 960mL H2O (or D2O for deuterated sample); adjust pH to 7.4 -> Autoclave  ||
|  Dissolve 8g glucose in 20mL H2O -> Filter  ||
|  8mL 1M MgSO4,  2mL 0.1M CaCl2 -> Autoclave  ||
|  6mL 1mM FeSO4 -> Filter  ||

Note: alternative: NaH2PO4 (monobasic) = 4.75g, Na2HPO4 (dibasic) = 3.73g, KH2PO4 (monobasic) = 7.15g

===== M9 (15N, 1L, with vitamin) =====
|Na2HPO4|12.8g|
|KH2PO4|3.0g|
|NaCl|0.5g|
|15N-NH4Cl|1g|
|  Add 1L H2O; adjust pH to 7.4 -> Autoclave  ||
|  Dissolve 8g glucose in 20mL H2O -> Filter  ||
|  Before using it, add Amp, 8mL 1M MgSO4, 2mL 0.1M CaCl2, 6mL 1mM FeSO4, 1mL of 10mg/mL Thiamine, 1mL of 10mg/mL Biotin and all the glucose solution.  ||


===== Nice practical guide on media for NMR isotope labeling =====
[[http://rainbow.life.tsinghua.edu.cn/~xuelab/protocol/protein_isotope/li_sykes.pdf|Li, Corson, and Sykes]]