====== Isotope chemicals ====== * http://www.silantes.com/ ====== TO ORDER (DNA template) ====== * WT-TAR: GGCAGAUCUGAGCCUGGGAGCUCUCUGCC


>>> Forward Sequence:
TTA ATA CgA CTC ACT ATA ggC AgA TCT gAg CCT ggg AgC TCT CTg CC

>>> Reverse Sequence:
ggC AgA gAg CTC CCA ggC TCA gAT CTg CCT ATA gTg AgT CgT ATT AA

* P5c: GGUGGCCUUGCAAAGGGUAUG


>>> Forward Sequence:
TTA ATA CgA CTC ACT ATA ggT ggC CTT gCA AAg ggT ATg

>>> Reverse Sequence:
CAT ACC CTT TgC AAg gCC ACC TAT AgT gAg TCg TAT TAA

* Hairpin01: GGAUUCGUGGCACUUCGGUGCUAUGAGUCC


>>> Forward Sequence:
TTA ATA CgA CTC ACT ATA ggA TTC gTg gCA CTT Cgg TgC TAT gAg TCC

>>> Reverse Sequence:
ggA CTC ATA gCA CCg AAg TgC CAC gAA TCC TAT AgT gAg TCg TAT TAA

====== TO ORDER (Reagents) ====== * Spermidine [已订] * PEG: Polyethylene glycol [暂不订] * MgCl2 [已订， by周桥] * DTT [已订, by宿锐] * DEPC [已订， by周桥] * DNA loading buffer [已订, by 宿锐] * RNA loading buffer[已订， by宿锐] * RNAse Inhibitor [已在NEB订购] * T7 RNA polymerase [找赵帅要/自提] * 50 mL falcon tube [已订， by 宿锐] * Urea [已订, by宿锐] * 1XTBE(RNAse free) [已订] * UV light [已订，by薛毅] * Na Acetate (3M, pH=5.2) [已订，by薛毅] * Tris [已订,by 宿锐] * 甘油 [已订] * 一次性手套 [已订,by 周桥] * EP tube [已订,by 周桥] * 保鲜膜 [已订,by 周桥] * 50 X TAE RNAse free [已订, 非RNAse free, by 周桥] * 0.2um Millipore Apparatus (to filter) * 量筒，三角瓶，烧杯，磨口三角瓶 [已订,by 周桥] * 15 mL falcon tube [已订,by 宿锐] * 电磁搅拌子 [已订,by 周桥] * 枪头 [已订,by 周桥] ====== TO ORDER (Equipments) ====== * Vortex两个 [已订] * 金属浴两个 [已订] * Mini离心机四个 [已订] * 知楚摇床 [已订] * 隔膜真空泵 [已订] * 高压细胞破碎机 [已订]