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====== General ======
<WRAP group>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>0.1M MgCl2</fc></fs>
  * 0.813g MgCl2 (MW 203.31)
  * 40mL H2O
  * filter
</WRAP>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>1M Tris</fc></fs>
  * 121g Tris base
  * 900mL H2O
  * filter
</WRAP>
</WRAP>

<WRAP group>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>1M DTT</fc></fs>
  * 1.543g DTT (MW 154.3)
  * 10mL H2O
</WRAP>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>APS, 10% w/v</fc></fs>
  * 5g Ammonium Persulfate (APS)
  * 50mL H2O
</WRAP>
</WRAP>

<WRAP group>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>0.25M Spermidine trihydrochloride</fc></fs>
  * 0.636g Sperm (MW 254.63)
  * 10mL H2O
  * filter
</WRAP>
</WRAP>

====== RNA/DNA Related ======
<fs x-large><fc #00ff00>100mM NTPs</fc></fs>
^rATP^rGTP^rCTP^UTP^Mix^
|276mg rATP (MW 551.1)|262mg rGTP (MW 523.2)|264mg rCTP (MW 527.1)|242mg UTP (MW 484.1)|rATP + rGTP + rCTP + UTP|
|5mL H2O|5mL H2O|5mL H2O|5mL H2O|5mL H2O|
|Add 50mM (500mM?) Tris Base to adjust pH to 7 (by pH paper) and filter|||||

<WRAP group>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>RNA Loading buffer, 50 mL</fc></fs>
  * 13 mg Bromphenol Blue
  * 10 mg Xylene cyanol
  * 930 mg EDTA
  * solve in 50 mL Formamide, heat briefly
</WRAP>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>DNA Loading buffer, 10 mL</fc></fs>
  * 25 mg Bromphenol Blue
  * 25 mg Xylene cyanol
  * 10mL 30% glycerol
</WRAP>
</WRAP>

<WRAP group>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>Agarose Gel</fc></fs>
  * 2g Agarose
  * 100mL 1X TAE
  * microwave until boiling
  * wait until warm and add 2.5uL EtBr
</WRAP>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>15% Polyacrylamide, 8M Urea, 1X TBE, 2L</fc></fs>
  * 960g Urea
  * 400mL 5X TBE
  * 750mL 40% Polyacrylamide
  * Stir over gentle heat
</WRAP>
</WRAP>

<WRAP group>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>Crush & Soak Buffer</fc></fs>
  * Tris 20mM
  * Sodium acetate 300mM
  * EDTA 1mM
</WRAP>
<WRAP half column round tip 40%>
<fs x-large><fc #00ff00>8M Urea, 1X TBE, 2L</fc></fs>
  * 960g Urea
  * 400mL 5X TBE
  * 1L DEPC H2O
</WRAP>
</WRAP>
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