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order
Table of Contents
Isotope chemicals
TO ORDER (DNA template)
- WT-TAR: GGCAGAUCUGAGCCUGGGAGCUCUCUGCC
>>> Forward Sequence: TTA ATA CgA CTC ACT ATA ggC AgA TCT gAg CCT ggg AgC TCT CTg CC >>> Reverse Sequence: ggC AgA gAg CTC CCA ggC TCA gAT CTg CCT ATA gTg AgT CgT ATT AA
- P5c: GGUGGCCUUGCAAAGGGUAUG
>>> Forward Sequence: TTA ATA CgA CTC ACT ATA ggT ggC CTT gCA AAg ggT ATg >>> Reverse Sequence: CAT ACC CTT TgC AAg gCC ACC TAT AgT gAg TCg TAT TAA
- Hairpin01: GGAUUCGUGGCACUUCGGUGCUAUGAGUCC
>>> Forward Sequence: TTA ATA CgA CTC ACT ATA ggA TTC gTg gCA CTT Cgg TgC TAT gAg TCC >>> Reverse Sequence: ggA CTC ATA gCA CCg AAg TgC CAC gAA TCC TAT AgT gAg TCg TAT TAA
TO ORDER (Reagents)
- Spermidine [已订]
- PEG: Polyethylene glycol [暂不订]
- MgCl2 [已订， by周桥]
- DTT [已订, by宿锐]
- DEPC [已订， by周桥]
- DNA loading buffer [已订, by 宿锐]
- RNA loading buffer[已订， by宿锐]
- RNAse Inhibitor [已在NEB订购]
- T7 RNA polymerase [找赵帅要/自提]
- 50 mL falcon tube [已订， by 宿锐]
- Urea [已订, by宿锐]
- 1XTBE(RNAse free) [已订]
- UV light [已订，by薛毅]
- Na Acetate (3M, pH=5.2) [已订，by薛毅]
- Tris [已订,by 宿锐]
- 甘油 [已订]
- 一次性手套 [已订,by 周桥]
- EP tube [已订,by 周桥]
- 保鲜膜 [已订,by 周桥]
- 50 X TAE RNAse free [已订, 非RNAse free, by 周桥]
- 0.2um Millipore Apparatus (to filter)
- 量筒，三角瓶，烧杯，磨口三角瓶 [已订,by 周桥]
- 15 mL falcon tube [已订,by 宿锐]
- 电磁搅拌子 [已订,by 周桥]
- 枪头 [已订,by 周桥]
TO ORDER (Equipments)
- Vortex两个 [已订]
- 金属浴两个 [已订]
- Mini离心机四个 [已订]
- 知楚摇床 [已订]
- 隔膜真空泵 [已订]
- 高压细胞破碎机 [已订]
order.txt · Last modified: 2016/11/26 18:00 by 127.0.0.1
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