0.1M MgCl2

• 0.813g MgCl2 (MW 203.31)
• 40mL H2O
• filter

1M Tris

• 121g Tris base
• 900mL H2O
• filter

1M DTT

• 1.543g DTT (MW 154.3)
• 10mL H2O

APS, 10% w/v

• 5g Ammonium Persulfate (APS)
• 50mL H2O

0.25M Spermidine trihydrochloride

• 0.636g Sperm (MW 254.63)
• 10mL H2O
• filter

RNA Loading buffer, 50 mL

• 13 mg Bromphenol Blue
• 10 mg Xylene cyanol
• 930 mg EDTA
• solve in 50 mL Formamide, heat briefly

DNA Loading buffer, 10 mL

• 25 mg Bromphenol Blue
• 25 mg Xylene cyanol
• 10mL 30% glycerol

Agarose Gel

• 2g Agarose
• 100mL 1X TAE
• microwave until boiling
• wait until warm and add 2.5uL EtBr

15% Polyacrylamide, 8M Urea, 1X TBE, 2L

• 960g Urea
• 400mL 5X TBE
• 750mL 40% Polyacrylamide
• Stir over gentle heat

Crush & Soak Buffer

• Tris 20mM
• Sodium acetate 300mM
• EDTA 1mM

8M Urea, 1X TBE, 2L

• 960g Urea
• 400mL 5X TBE
• 1L DEPC H2O